Green modified-UiO-۶۶/MXene sandwich composites for gene chemotherapysynergistic cancer suppression: Co-delivery of doxorubicin and pCRISPR

Advancements in material science and engineering, especially in the field of porousnanomaterials lead to new types of therapeutics and technologies. Among different types of porousnanomaterials, metal-organic frameworks (MOFs) have been considered the most promisinginorganic nanomaterials due to their significant stability and ease of functionalization. To date,very limited research has been done on developing nanocomposites based on MOFs and MXenes.The current experiment has been performed to design a novel UiO-۶۶ MOF and MXenenanocomposite, modified with Rosmarinus officinalis (RO) leaf extracts for co-delivery ofdoxorubicin (DOX)/plasmid (p)CRISPR ([۱]). The synthesis procedure was completed with theassistance of a high-gravity system and a rotating packed bed (RPB) device for the first time, andthe characterization results showed the successful synthesis of the nanomaterials. Based on thedrug release and loading results, the DOX payload efficiency of about ۴۶% was recorded andsustained drug release at acidic pH values (same as the cancerous cells) was observed. However,the presence of leaf extracts slowed down the drug release and have a minor effect on the releaseprofiles, the cellular internalization results on the MCF-۷ and HT-۲۹ cell lines showedconsiderably dependent images. The gene transfections result by using two nanosystems of UiO-۶۶@MXene@RO@DOX@pCRISPR, and UiO-۶۶@MXene@RO@DOX@RO@pCRISPRshowed about ۱۸.۳% and ۲۲.۱% transfection efficiency on the A۵۴۹ cell lines. Those resultsshowed not heavily dependent results on the presence or absence of the leaf extracts. Therefore,this study revealed that the green modification of nanocomposites does have a major effect onlyon the cellular internalizations of DOX, rather than CRISPR ([۲]).

کلیدواژه ها:

MOF ، MXene ، doxorubicin ، CRISPR ، leaf extracts