Natural Deoxynivalenol Contamination of Corn Produced in Golestan and Moqan Areas in Iran
محل انتشار: مجله علوم و فناوری کشاورزی، دوره: 12، شماره: 2
سال انتشار: 1389
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 36
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شناسه ملی سند علمی:
JR_JASTMO-12-2_011
تاریخ نمایه سازی: 1 آذر 1402
چکیده مقاله:
Deoxynivalenol contamination was determined in corn produced in Golestan and Ardabil (Moqan) Provinces, Iran, in ۲۰۰۴-۲۰۰۵. Samples were collected from different stages of production, including before harvest, at harvest, post harvest as well as after drying. Ground sub samples were extracted with water; each extracted sample was cleaned up through an immunoaffinity column. Deoxynivalenol was estimated through reversed-phase High Performance Liquid Chromatography (HPLC). The linearity of standard curve for ۵۰ -۱۰,۰۰۰ ng ml-۱ of standard solutions was proved (R۲= ۰.۹۹۹۹). Detection limit was ۱۰ ng g-۱. Recovery of the method for ۱,۰۰۰ and ۵۰۰ ng g-۱ spiked samples was ۷۳.۵% and ۹۳.۵% (n= ۵). Deoxynivalenol contamination was found in ۷۶.۷% of samples in the range of ۵۴.۴-۵۱۸.۴ ng g-۱. The mean of contamination was ۱۱۶.۲۵ ng g-۱. This is the first report of natural DON contamination of corn from Iran.
کلیدواژه ها:
نویسندگان
R. Karami-Osboo
Mycotoxins Research Laboratory, Plant Pests and Diseases Research Institute, Tehran, Islamic Republic of Iran.
M. Mirabolfathi
Mycotoxins Research Laboratory, Plant Pests and Diseases Research Institute, Tehran, Islamic Republic of Iran.
F. Aliakbari
Islamic Azad University, Tehran, Islamic Republic of Iran.