Investigation of Plant Growth Regulators Effects on Callus Induction and Shoot Regeneration of Bunium persicum (Boiss.) B. Fedtsch.
محل انتشار: مجله علوم و فناوری کشاورزی، دوره: 11، شماره: 4
سال انتشار: 1388
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 59
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شناسه ملی سند علمی:
JR_JASTMO-11-4_011
تاریخ نمایه سازی: 1 آذر 1402
چکیده مقاله:
Parsi Zira, Bunium persicum (Boiss.) B. Fedtsch., or Black Zira, a member of the Umbellifera family, is one of the most important medicinal species with high economic value. Generally, there is limited information about in vitro culture of Bunium persicum. In this study, the effect of various media (MS, B۵) and combinations of plant growth regulators, NAA and ۲,۴-D alone or together with Kin, on callus induction and shoot regeneration from hypocotyl explant were investigated. This experiment has been carried out in a completely randomized design with ۳۰ treatments and ۱۰ replications per treatment. The results showed significant effects of treatments on regeneration and callus induction. All the measured traits for MS medium were superior. The highest callus frequency was observed on the medium containing ۰.۱ mg L-۱ or ۱ mg L-۱ ۲,۴-dichlorophenoxyacetic acid (۲,۴-D) as well as ۲ mg L-۱ ۲,۴-D and ۰.۵ mg L-۱ kinetin. The best treatment for somatic embryogenesis was the medium containing ۲ mg L-۱ ۲,۴-D. The best response for shoot regeneration was observed on the medium supplemented with ۱ mg L-۱ ۲,۴-D. The medium containing ۱ mg L-۱ ۲,۴-D was the best for callus induction and shoot regeneration simultaneously. The regenerated shoots were rooted on basal medium.
کلیدواژه ها:
نویسندگان
M. Valizadeh
Department of Agronomy and Plant Breeding, Faculty of Agriculture, Mazandaran University, Mazandaran, Islamic Republic of Iran
S. K. Kazemi Tabar
Department of Agronomy and Plant Breeding, Faculty of Agriculture, Mazandaran University, Mazandaran, Islamic Republic of Iran