Identification of AFLP Marker Associated with Stress Tolerance Index in Sardari Wheat Ecotypes

Sardari is one of the most important landraces of common wheat (Triticum aestivum L.) that is mainly cultivated in drylands and mountainous area of Iran. Moreover, it shows a high level of genetic diversity. In the present research a total of ۷۳ Sardari wheat ecotypes were evaluated for drought tolerance. Genetic diversity was analyzed using amplified fragment length polymorphism (AFLP) marker based on three pairs of primer combinations. Of the ۲,۴۳۱ AFLP bands detected, ۱,۵۸۲ (۷۳.۹۲%) were polymorphic. Cluster analysis divided all ecotypes into eight major groups. Ecotypes also showed genetic diversity for drought tolerance and were classified into three groups. The first group consisted of forty-two of the ۷۳ landraces and had a low stress tolerance index (STI), ranging from ۰.۱۶۵ to ۰.۴۰۱, while the second (۲۳ landraces) and the third group (۷ landraces) had a medium and high STI ranging from ۰.۴۲۵ to ۰.۶۴۰ and ۰.۶۶۲ to ۰.۸۱۷, respectively. Discriminant analysis (DA) identified twenty-four markers selected from ۲۱۸ AFLP markers that accounted for the difference between the three phenotypic groups. By using the selected markers, DA validated the phenotypic grouping, with a zero error rate. The results showed a high degree of genetic diversity between the Sardari ecotypes, suggesting that Sardari can be used as a germplasm source for wheat improvement toward releasing more desirable cultivars.

کلیدواژه ها:

AFLP ، Discriminate analysis ، genetic diversity ، Sardari wheat ، STI