Resistance Mechanisms to Chlorpyrifos in Iranian Populations of the Two-spotted Spider Mite, Tetranychus urticae (Acari: Tetranychidae)
محل انتشار: مجله علوم و فناوری کشاورزی، دوره: 16، شماره: 2
سال انتشار: 1392
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 84
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شناسه ملی سند علمی:
JR_JASTMO-16-2_003
تاریخ نمایه سازی: 1 آذر 1402
چکیده مقاله:
The toxicity of chlorpyrifos to three Iranian populations of two-spotted spider mite, collected from Isfahan (ISR), Yazd (Yz) and Guilan (GUS۲) Provinces were surveyed using the residual contact vial bioassay. The bioassay results showed that resistance ratios of ISR and Yz populations were ۱۷۶.۹۰ and ۹.۷۸ fold compared to the GUS۲ population, respectively. Determination of esterase and glutathione-S-transferase activity and their kinetic parameters showed that ISR population had the highest specific activity and specificity constant among the studied populations. Besides, the content of mixed function oxidases in ISR population was the highest. However, synergistic effects of Piperonyl Butoxide, Diethyl Maleate and Triphenyl Phosphate showed that metabolic enzymes did not play an important role in resistance to chlorpyrifos in ISR and Yz populations and enhanced activity of esterase, glutathione-S-transferase and content of mixed function oxidases in these populations were probably due to resistance to some other acaricides. To determine the role of acetylcholinesterase insensitivity in resistance mechanisms, kinetic parameters and inhibitory effect of chlorpyrifos-oxon on this enzyme were investigated. The Km value of acetylcholinesterase was determined as ۰.۰۳۶, ۰.۰۴, and ۰.۰۵۰ mM using acetylthiocholine iodide for GUS۲, Yz, and ISR populations, respectively. In addition, the insensitivity ratios of chlorpyrifos-oxon on acetylcholinesterase activity were estimated at ۲۳.۳۰ and ۲.۹۶ for ISR and Yz populations, respectively. These results confirmed amino acid substitutions in active site of this enzyme and also indicated that resistant population possed qualitatively altered AChE.
کلیدواژه ها:
نویسندگان
P. Zamani
Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Islamic Republic of Iran.
R. H. Sajedi
Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Islamic Republic of Iran.
M. Ghadamyari
Department of Plant Protection, Faculty of Agriculture, University of Guilan, Rasht, Islamic Republic of Iran.
N. Memarizadeh
Department of Plant Protection, Faculty of Agriculture, University of Guilan, Rasht, Islamic Republic of Iran.
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