Identification of Tobacco Leaf Curl Virus Infecting Lonicera japonica, an Ornamental Plant Common in Japan

A begomovirus, tentatively named Tobacco leaf curl Japan virus-JpU (TbLCJV-JpU), was isolated from Lonicera japonica (Honeysuckle) Plants Grown in Utsunomiya showing veinal chlorosis symptoms. The TbLCJV-JpU genome with ۲,۷۶۱ nt showed a highest identity with TbLCJV-Jp۳ and was also close to TbLCJV and as well to TbLCJV-Jp۲. The overall nt identity with TbLCJV-Jp۳ amounted to ۹۲.۹۴%, while the identities in encoded amino acid (aa) sequence of Coat Protein (CP) and putative products of AC۱ and AV۲ ORFs were as high as ۹۸.۰۵, ۹۲.۵۴ and ۹۳.۹۶%, respectively. Low sequence identities were observed in the Intergenic Region (IR) of TbLCJV-JpU as compared with TbLCJV, Ageratum yellow vein Taiwan virus-Kochi isolate and Honeysuckle yellow vein virus-Kagoshima isolates. Recombinations were detected in the ۵´end (۲۶۵۰ to ۲۷۶۱) and extreme ۳´ portion of the genome (۲۲۰ to ۳۵۰). Both regions demonstrated high identities with AYVTV-Kochi and HSYVV-Kagoshima. To the best of our knowledge this is the first report of isolation of TbLCJV from L. japonica.

کلیدواژه ها:

Begomovirus ، Japan ، Lonicera japonica ، Tobacco leaf curl Japan virus