Occurrence, Distribution, and Molecular Characterization of Apple Stem Pitting Virus in Iran

سال انتشار: 1395
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 115

فایل این مقاله در 14 صفحه با فرمت PDF قابل دریافت می باشد

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:

شناسه ملی سند علمی:

JR_JASTMO-19-1_018

تاریخ نمایه سازی: 1 آذر 1402

چکیده مقاله:

Apple Stem Pitting Virus (ASPV) is one of the most common viruses of apple and pear trees. During ۲۰۱۲ to ۲۰۱۳, a total of ۱۰۵۳ symptomatic and asymptomatic leaf samples were collected from orchards in nine provinces of Iran. ASPV infection was detected by DAS-ELISA and PCR in ۵۴ samples (۵.۱۲%) from seven provinces. Based on the geographical origin, ۲۲ representative isolates were selected for phylogenetic analysis. Twenty-two amplicons with a size of about ۳۷۰ base pair (bp) comprising partial sequence of the Coat Protein (CP) coding regions of the viral genome were sequenced. Sequence data analysis, showed that the identities of ۳'-terminal region of CP gene at both nucleotide and amino acid levels among the Iranian isolates were ۹۵–۱۰۰% and these isolates were closer to the Asian ASPV isolates than to the other isolates. Constructed phylogenetic tree by Neighbor-joining on the basis of the ۳'-terminal region of CP gene sequences showed that the Iranian isolates were categorized into two major groups. Furthermore, phylogenetic and population genetic analysis were carried out on the basis of ۳'-terminal region of CP gene which revealed that ASPV isolates were not geographically resolved. Also, all values in the GABranch analysis showed a ratio of substitution rates at Non-synonymous and Synonymous sites (dN/dS) below one, suggesting strong negative selection forces during C-terminal region of the ASPV CP gene history. To the best of our knowledge, this is the first report of distribution and partial genome sequence analysis of the ASPV in Iran.

نویسندگان

F. Abtahi

Plant Pathology Department, Faculty of Agriculture, Tarbiat Modares University, Tehran, Islamic Republic of Iran.

M. Shams-bakhsh

Plant Pathology Department, Faculty of Agriculture, Tarbiat Modares University, Tehran, Islamic Republic of Iran.

N. Safaie

Plant Pathology Department, Faculty of Agriculture, Tarbiat Modares University, Tehran, Islamic Republic of Iran.

C. R. Autonell

Plant Pathology Department, Faculty of Agriculture, University of Bologna, P. O. Box: ۴۰۱۲۷, Bologna, Italy.

C. Ratti

Plant Pathology Department, Faculty of Agriculture, University of Bologna, P. O. Box: ۴۰۱۲۷, Bologna, Italy.

مراجع و منابع این مقاله:

لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :
  • Bao, Y., Chetvernin, V. and Tatusova, T. ۲۰۱۲. Pairwise Sequence ...
  • Bao, Y., Chetvernin, V. and Tatusova, T. ۲۰۱۲. Pairwise Sequence ...
  • نمایش کامل مراجع