Pathway analysis using microarray dataof PC-۳ cells following MS۱۳ treatment

MS۱۳ is a curcumin analogue which has been proven to havegreater anti-cancer effect on PC-۳ and DU ۱۴۵ human prostatecancer cells. The MS۱۳ has been recognized for its potentialas an anti-tumor agent against various types of cancer cells,including gastric, cervical, and prostate cancers. The currentresearch aims to improve the understanding of the antitumormechanisms of MS۱۳. To achieve this, a previously publisheddataset was reanalyzed, which involved the treatment of PC-۳cells with MS۱۳.A collection of ۱۲ samples (GSE۱۷۹۳۲۴) was obtained fromthe Gene Expression Omnibus. After assessing the statisticalparameters, ۶ samples including ۳ control and ۳ MS۱۳ treatedPC-۳ cells were chosen for GEO۲R analysis. Data were thenanalyzed using the Benjamini & Hochberg method and a Significancelevel of ۰.۰۵. Differentially expressed genes with |logFold Change| > ۱ and adjusted p-value < ۰.۰۵ were identifiedusing R programming. Additionally, a Volcano plot and heatmapwere created to visualize the selected DEGs. Subsequently,the upregulated and downregulated genes were used for enrichmentanalysis in the Enrichr database.The study deemed ۱۶۸ genes as differentially expressed genesincluding ۳۲ upregulated and ۱۳۶ downregulated genes. Thepathway analysis revealed the involvement of several of theseselected genes in the pancreatic cancer pathway.In the context of pancreatic cancer, the downregulation ofRAC۲ and RAC۳ genes can lead to a reduction in NF-κB activation,resulting in the inhibition of Anti-apoptotic genes.This, coupled with a decrease in cytoskeleton remodeling, caneffectively reduce the aggressiveness of pancreatic cancer. Additionally,if TGFBR۲, SMAD۲, and SMAD۳ genes involved inthe TGF-β signaling pathway are downregulated in pancreaticcancer cells, it could prevent the loss of growth inhibitory effectof TGF-β. In light of the aforementioned assumptions and theproven effectiveness of curcumin in treating pancreatic cancer,it is recommended that future experimental studies evaluate thepotential anticancer properties of MS۱۳ specifically for thistype of cancer.

کلیدواژه ها:

MS۱۳ ، Pancreatic cancer ، Pathway analysis ، Prostatecancer ، TGF-β signaling pathway