miR-۲۹b-۳p regulates MOB۱A/B in Tannicacid treated K۵۶۲-CML cell line

Introduction: MicroRNAs are small, highly conserved noncodingRNA molecules involved in the regulation of gene expressioninvolved in various developmental and pathophysiologicalprocesses including cancer. It is established that Hipposignaling can regulate cell proliferation, differentiation, apoptosis,and regeneration. Any dysregulation of Hippo signalingcan lead to uncontrolled cell proliferation and malignant transformation.Recent studies have shed new light on the regulatoryrole of microRNAs in Hippo signaling and how they contributeto cancer progression. In recent years, miR-۲۹ has emerged as acritical miRNA in various cancers, and it has been shown to regulatemultiple oncogenic processes. Although miR-۲۹ has beenthoroughly documented as a tumor suppressor in the majorityof studies, some controversy remains with conflicting reportsof miR-۲۹ as an oncogene. Tannic acid (TA), a phytochemicalagent, is a common ingredient in many foods. Recently, the cellcycle arrest, apoptosis induction, reduced rate of proliferation,and cell migration of several cancer cell lines as a result of TAtreatment were described. In this study, we are aimed to surveythe miR-۲۹b-۳p and Hippo signaling pathway gene expressionpatterns in TA-treated K۵۶۲ cells.Methods: K۵۶۲ cells were treated with TA and then cell proliferationwas assayed with trypan blue staining. Moreover, theexpression of miR-۲۹b-۳p and MOB۱A/B, the upstream elementsof Hippo pathway, were evaluated by real-time PCR.Results: Treatment with TA decreased cell proliferation in aconcentration-dependent manner and, ۵۰% inhibition of cell viabilitywas obtained at ۱۰μM. Also, down-regulation of miR-۲۹b-۳p along with up-regulation of MOB۱A/B expression levelswas observed in treated cells compared to control cells.Conclusion: Our results may help to shed light on the mechanismby which TA modulates cell proliferation in K۵۶۲ cell line.

کلیدواژه ها:

MicroRNA ، Hippo signaling pathway ، Tannic acid ، MOB۱A/B