Comprehensive analysis of GUCA۲A as aprognostic and diagnostic marker in colorectal cancer

Background: Cancers of all types are the primary cause ofdeath globally and one of the most significant obstacles to increasedlife expectancy. Herein, we aimed to determine a significantcolorectal cancer-specific differentially expressed gene.Materials and Methods: Gene expression analysis of fourcolorectal cancer microarray datasets in the GEO databasewere identified differentially expressed genes (DEGs). Next,the TCGA colon adenoma carcinoma (COAD) dataset was investigatedusing GEPIA۲ to determine all COAD-associatedDEGs among high throughput RNA-Seq data. Common DEGsbetween the GEO datasets and the GEPIA۲ COAD-TCGA dataanalyzed in pan cancer model among ۳۳ cancer types. Gene setenrichment analysis was performed using Enrichr. Then, coexpressionnetwork was constructed via STRING and LinkedOmicsdatabases, respectively. Finally, the competing endogenousRNA (ceRNA) network was constructed based on our in-silicoanalyses using data from miRTarBase and circBank databases.Results: Finally, GUCA۲A was found as a significant colorectalcancer-specific differentially expressed gene (|log۲FC| >۱ and adjusted q-value < ۰.۰۵). More remarkably, with ۹۸%sensitivity, ۹۵% specificity and ۹۹.۶% AUC, GUCA۲A canbe used as a diagnostic marker for COAD. Furthermore, lowexpression of GUCA۲A was significantly affected on patient’soverall survival. Enrichment analysis defined receptor guanylylcyclase signaling pathway and guanylate cyclase activator activityas the most significant gene ontology terms. Additionally,a ceRNA network containing ۸ miRNAs targeting GUCA۲Aand ۱۸۳ circRNAs as miRNA sponges was constructed. Lactoseanhydrous, Atropin, and Volanesorsen sodium were identifiedas drugs having interaction with GUCA۲A.Conclusion: Overall, Present study deciphers GUCA۲A as aprognostic and diagnostic biomarker in colorectal cancer. Furtherstudies need to be done regarding GUCA۲A as a therapeuticbiomarker.

کلیدواژه ها:

Colorectal cancer ، GUCA۲A ، Prognostic biomarker ، Diagnostic biomarker ، CeRNA network ، Integrated Bioinformaticanalysis