Ethephon causes reproductive malfunction in adult male mice: Histological and biochemical evidence
محل انتشار: گفتمان پژوهش دامپزشکی، دوره: 12، شماره: 3
سال انتشار: 1400
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 87
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شناسه ملی سند علمی:
JR_VRFAN-12-3_011
تاریخ نمایه سازی: 27 آبان 1402
چکیده مقاله:
Ethephon (C۲H۶ClO۳P; ETP), an organophosphorus pesticide regulating plant growth, is widely used for early ripening of fruits and vegetables in agriculture. The aim of this study was to investigate the effects of ETP on histomorphometrical and biochemical parameters in mouse testicular tissue. In this study, ۹۰ adult male mice were randomly divided into six equal groups (n = ۱۵). The ETP was administered orally at different doses (۱۲۰, ۲۴۰ and ۴۸۰ mg kg-۱) daily for ۳۵ days. Untreated control, sham (received only normal saline) and neostigmine bromide-treated (positive control; ۰.۱۰ mg kg-۱ orally; once per week) groups were also considered. Following ۳۵ days, animals were euthanized and testicle and serum samples were taken. Accordingly, blood and serum acetylcholinesterase (AChE), catalase, total antioxidant capacity (TAC) and malondialdehyde (MDA) levels as well as histomorphometrical changes of testicles were investigated. The ETP-administered animals represented a significant reduction in AChE, TAC and catalase levels and remarkable increment in MDA content. A marked reduction was also seen in the germinal epithelium height, connective tissue thickness, seminiferous tubules diameter and Leydig cell number as well as spermiogenesis and Sertoli cell indices in ETP-treated mice compared to control ones. Similar findings were found in neostigmine bromide-treated animals. In conclusion, the ETP significantly affects the serum and blood anti-oxidant statuses and results in severe histological damages both at germ and somatic cell levels, suggesting its hematotoxic and reprotoxic characteristic.
کلیدواژه ها:
نویسندگان
Ramin Jahangirfard
PhD Candidate, Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Gholamreza Najafi
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Ali Shalizar-Jalali
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Abbas Ahmadi
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Elham Zadehhashem
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
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