Valuable Modern Strategy (ATR-IR) Spectroscopy Technique to Distinguish Between Normal and Lung Cancer Tissue

Background: Diagnosis of lung cancer is often delayed because most of the patients are asymptomatic during the primary cancer stages. Infrared spectroscopy is an improved technique compared with the others for identifying abnormal tissue types because of its spatial and spectral capabilities. Therefore, this study is taking an advanced advantage of the physical and optical properties of the attenuated total reflection Fourier-transform infrared system.Methods: The attenuated total reflection Fourier-transform infrared has been applied for cancer detection at infrared wavelengths that range from ۴۰۰۰-۴۰۰ cm-۱. This technique may be a beneficial diagnostic method because it uses the principles of physics, as optics and photonics with a specific wavelength region, which can make an immense difference.Results: The attenuated total reflection Fourier-transform infrared system spectra of normal lung tissue showed main peaks at ۳۳۲۱ and ۱۶۳۷ cm-۱, which has been assigned to the OH and C=O function group of amide I and has intensities of approximately ۶۱% (OH) and ۷۶% (C=O). That intensity has been shown to decrease in the cancer tissue. A new peak at ۱۵۴۵ cm-۱ appeared in the cancerous tissue, which could be an amide II.Conclusions: The identification of a biochemical component from either normal or cancerous lung tissue would help to evaluate malignant tissue. Thus, the obtained results indicated degradation of the biochemistry component (protein) of the tissue due to carcinogenic disease.