Evaluation of the Methylation Status of the MEIS۱ Promoter Gene in Colorectal Cancer

Background: Colorectal cancer, the third most common type of cancer is a major cause of mortality worldwide. If colorectal cancer is detected at the early stages, the ۵-year survival rate is ۹۰%. MEIS۱ homeobox gene is implicated in numerous solid tumors and hematological malignancies. Therefore, the present study aims to investigate the methylation status of the MEIS۱ gene in colorectal cancer.Methods: We used real-time quantitative methylation-specific PCR to detect MEIS۱ promoter methylation in ۴۲ colorectal cancer tissues and ۴۲ normal colorectal tissues.Results: Methylation was observed only in the positive control samples - CG Genome Universal Unmethylated DNA and CG Genome Universal Methylated DNA. There was no change observed in MEIS۱ promoter methylation status in ۴۲ patients.Conclusion: The results of the current study indicated that the MEIS۱ gene promoter was not methylated in the cases. Gene expression study confirmed the unmethylated status of the MEIS۱ gene in the colorectal cancer process among the studied population.