CTLA-۴ Exon One +۴۹A/G Gene Variants in Patients with Superficial and Invasive Bladder Cancer: A Study in Southern Iran
محل انتشار: مجله سرطان خاورمیانه، دوره: 1، شماره: 1
سال انتشار: 1389
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 114
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شناسه ملی سند علمی:
JR_MISJ-1-1_003
تاریخ نمایه سازی: 25 آبان 1402
چکیده مقاله:
Introduction: Cytotoxic T-cell lymphocyte antigen ۴ (CTLA-۴) is a member of the superfamily of immunoglobulins that are mainly expressed by activated T cells. It is established that blockade of CTLA-۴ receptors leads to the enhancement of an immune response. Different polymorphisms of the CTLA-۴ gene have been described which cause increased susceptibility to various malignancies such as lymphoma or multiple myeloma. Considering that bladder cancer is one of the most prevalent cancers worldwide, we have evaluated the role of CTLA-۴ gene polymorphism at position +۴۹ A/G in the formation or progression of bladder cancer in southern Iran.Materials and Methods:Atotal of ۲۲۶ individuals between February ۲۰۰۵ and June ۲۰۰۶ were included and placed into two subgroups: patients diagnosed with bladder cancer and a control group. Demographic data and risk factors were collected from both groups. The DNAof all subjects was extracted from their blood samples. Different genotypes of the CTLA-۴ gene were determined using the restriction fragment length polymorphism (RFLP) technique and data were compared in both groups by using Pearson's chi-square test.Results: The prevalence of AA, AG and GG genotypes at position ۴۹, according to the PCR-RFLP method, were ۵۷.۵%, ۳۷.۲% and ۵.۳% in the control group, respectively. In the patient group, the prevalence of these genotypes was: AAin ۵۷.۵%, AG in ۳۲.۷% and GG in ۹.۸%. Statistical analysis of data showed no significant difference in both groups (P value=۰.۴۰). Also there was no correlation between different genotypes of the CTLA-۴ gene and invasiveness of the disease in our cases.Conclusion: Although polymorphism of the CTLA-۴ gene at position ۴۹ of exon-۱ increases susceptibility to several malignancies, our study showed no relationship between polymorphism at this position and genetic susceptibility to the development of bladder cancer, nor was there any association with disease progression.
نویسندگان
Alireza Aminsharifi
Department of Urology, Shiraz University of Medical Sciences, Shiraz, Iran
Abbas Ghaderi
Shiraz Institute for Cancer Research, Shiraz University of Medical Sciences, Shiraz, Iran
Sirous Naeimi
Shiraz Institute for Cancer Research, Shiraz University of Medical Sciences, Shiraz, Iran
Abdolaziz Khezri
Department of Urology, Shiraz University of Medical Sciences, Shiraz, Iran
Neda Agahi
Department of Urology, Shiraz University of Medical Sciences, Shiraz, Iran
Amir Aminian
Department of Urology, Shiraz University of Medical Sciences, Shiraz, Iran
Nima Zamiri
Health Policy Research Center, Shiraz University of Medical Sciences, Shiraz, Iran