An ethyl acetate fraction of flavonoids from Polygonum hydropiper L. exhibits an anti-inflammatory activity in PCV۲-infected porcine alveolar macrophages via PI۳K/Akt and NF-κB pathways

Porcine circovirus type ۲ (PCV۲) widely exists in swine production systems causing porcine circovirus diseases (PCVD) which is associated with significant economic losses. Polygonum hydropiper L. was used as a traditional Chinese medicine to treat a variety of diseases. This study was carried out to investigate anti-inflammatory activity of the ethyl acetate fraction of flavonoids from Polygonum hydropiper L. (FEA) in PCV۲-induced porcine alveolar macrophages (۳D۴/۲ cell line). The production of oxygen species (ROS) and the levels of tumor necrosis factor-α (TNF-α), interleukin-۱β (IL-۱β) and interleukin-۸ (IL-۸) were detected to evaluate the anti-inflammatory activities of FEA. The translocation of nuclear factor-kappa B (NF-κB) and the phosphatidylinositol ۳ kinase/protein kinase B (PI۳K/Akt) signaling pathways were investigated to document the potential anti-inflammatory mechanisms. In PCV۲ induced ۳D۴/۲ cells, FEA treatment significantly reduced the production of ROS, and sharply down-regulated the levels of TNF-α, IL-۱β and IL-۸ in both secretion and mRNA expression level. The FEA also decreased the mRNA expression of Akt and NF-κB p۶۵, reduced the transfer of p۶۵ to nuclear, and inhibited the activation of PI۳K/Akt signaling pathway. The findings suggest that FEA exhibited an anti-inflammatory activity in vitro and could be used as a candidate in treatment of inflammation induced by PCV۲ infection.

کلیدواژه ها:

۳D۴/۲ cell ، Anti-inflammatory activity ، Polygonum hydropiper L ، Porcine circovirus type ۲