Immunohistochemical determination of somatostatin release in gastric tissue of rats fed with a high-fat and cholesterol diet
محل انتشار: گفتمان پژوهش دامپزشکی، دوره: 14، شماره: 6
سال انتشار: 1402
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 42
فایل این مقاله در 7 صفحه با فرمت PDF قابل دریافت می باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_VRFAN-14-6_002
تاریخ نمایه سازی: 25 آبان 1402
چکیده مقاله:
This study aimed to investigate the effects of a high-fat and cholesterol diet (HFCD) on rats gastric mucosa. In the study, a total of ۱۶ (۴۰-day-old Sprague Dawley) male rats were used and randomly divided into two groups (each consisted of eight rats). Rats in the control group had no implementations other than normal feeding. For ۱۰ weeks, rats in a high-fat with cholesterol diet group had daily energy amounts provided by pellet feed mixed with ۶۵.۰۰% butter and ۲.۰۰% cholesterol. Before beginning the study and at the end, rats live weight was recorded and their blood samples were taken for biochemical analyses. Hematoxylin and Eosin and Crossman’s triple staining techniques were used to investigate the general structure of gastric tissue. Rats fed with HFCD had statistically significant increases in live weight and total cholesterol values, and were identified to have gastric tissue degeneration. The rats gastric tissue in control group had more intense somatostatin (SST) immunoreactivity in parietal and chief cells than the HFCD group. It was determined that feeding with the HFCD has a negative effect on SST secretion in rats and hence, this may have important areas of use such as in gastric cancer treatment and preventing complications linked to gastric diseases.
کلیدواژه ها:
نویسندگان
Habibe Gündoğdu
Department of Histology and Embryology, Faculty of Medicine, Ataturk University, Erzurum, Türkiye
Ebru Karadağ Sari
Department of Histology and Embryology, Faculty of Veterinary Medicine, Kafkas University, Kars, Türkiye
مراجع و منابع این مقاله:
لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :