In vitro sperm storage with poultry oviductal secretions

In the hen oviduct, tubules have been identified that preserve the sperm, maintaining viability for up to ۱۵ weeks. This study aimed to evaluate the physiological status of rooster sperm when preserved in vitro with uterus vaginal junction secretions (UVJS). Males and females of the Rhode Island breed were used. Sperm aliquots were prepared using Lake extender and Lake extender with UVJS (۱۰.۰۰%, ۳۰.۰۰%, ۶۰.۰۰%, and ۹۰.۰۰%). Subsequently, a basic sperm evaluation was performed and sperm physiological status was determined through the presence and distribution of Ca۲+ and its acrosomal reaction capability via perivitelline layer (PVL) co-incubation. It was observed that motility was decreased in sperm preserved with UVJS at ۶ and ۲۴ hr) compared to ۴۰ min and fresh semen. The sperm decapacitation percentage was increased when preserved with UVJS at ۴۰ min, ۶ and ۲۴ hr compared to fresh semen. The acrosomal reaction was increased in sperm co-incubated with PVL, even when preserved with UVJS. It was concluded that UVJS induced physiological changes in sperm by inducing a decapacitation process, which increased sperm viability when preserved in vitro.

کلیدواژه ها:

Acrosome ، decapacitation ، Hen ، oviduct ، Semen