Molecular characterization of a new microvariant of the G۳ genotype for Echinococcus granulosus in water buffalo in Iran

In this study, molecular characterization of Echinococcus granulosus sample obtained from water buffalo originating from southwest of Iran was performed using comparative sequence analysis of cox۱ mitochondrial gene. DNA was extracted from protoscoleces removed from hydatid cyst from the liver of a ۲-year-old male buffalo slaughtered in Khuzestan province. Molecular and phylogenetic analyses were conducted based on cox۱ mitochondrial gene. We found the presence of a new microvariant of G۳ genotype for E. granulosus in Iran which isgenetically differentiated from reference G۳ sequence (M۸۴۶۶۳).The difference was a transition mutation of adenine to guanine in position ۲۱۴ (A۲۱۴G) resulting in a substitution of the threonine (ACT) by alanine (GCT). These findings extend the knowledge of heterogeneity and distribution of G۳ genotype for E. granulosus in world.

کلیدواژه ها:

Echinococcus granulosus ، G۳ genotype ، Iran ، Mitochondrial gene ، Water buffalo