Protective effect of ethyl pyruvate on mice sperm parameters in phenylhydrazine induced hemolytic anemia
محل انتشار: گفتمان پژوهش دامپزشکی، دوره: 7، شماره: 1
سال انتشار: 1395
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 73
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شناسه ملی سند علمی:
JR_VRFAN-7-1_010
تاریخ نمایه سازی: 25 آبان 1402
چکیده مقاله:
The aim of the present study was to assess the protective effect of ethyl pyruvate (EP) on sperm quality parameters, testosterone level and malondialdehyde (MDA) in phenylhydrazine (PHZ) treated mice. For this purpose, ۳۲ NMRI mice with the age range of ۸ to ۱۰ weeks, weight average ۲۶.۰ ± ۲.۰ g, were randomly divided into four equal groups. The control group (۱) received normal saline (۰. ۱ mL per day) by intraperitoneal injection (IP). Group ۲ (PHZ group) was treated with initial dose of PHZ (۸ mg ۱۰۰ g-۱, IP) followed by ۶ mg ۱۰۰ g-۱ , IP every ۴۸ hr. Group ۳, (Group PHZ+EP) received PHZ (according to the previous prescription) with EP (۴۰ mg kg-۱, daily, IP). Ethyl pyruvate group (۴) received only EP (۴۰ mg kg-۱, daily, IP). Treatment period was ۳۵ days. After euthanasia, sperms from caudal region of epididymis were collected and the total mean sperm count, sperm viability, motility and morphology were determined. Testis tissue MDA and serum testosterone levels of all experimental groups were also evaluated. A considerable reduction in mean percentage of number, natural morphology of sperm, sperm motility and viability and serum testosterone concentration besides DNA injury increment among mice treating with PHZ in comparison with control group were observed. However, in PHZ+EP group the above mentioned parameters were improved. This study showed that PHZ caused induction of toxicity on sperm parameters and reduction of testosterone as well as the increment of MDA level and EP as an antioxidant could reduce destructive effects of PHZ on sperm parameters, testosterone level and lipid peroxidation.
کلیدواژه ها:
نویسندگان
Ali Akbar Mozafari
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Rasoul Shahrooz
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Abbas Ahmadi
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Hassan Malekinjad
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Karim Mardani
Department of Food Hygiene and Quality control, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
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