Effects of in vitro selenium addition to the semen extender on the spermatozoa characteristics before and after freezing in water buffaloes (Bubalus bubalis)
محل انتشار: گفتمان پژوهش دامپزشکی، دوره: 3، شماره: 4
سال انتشار: 1391
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 38
فایل این مقاله در 6 صفحه با فرمت PDF قابل دریافت می باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_VRFAN-3-4_007
تاریخ نمایه سازی: 25 آبان 1402
چکیده مقاله:
The aim of the present study was to investigate the effect of in vitro supplementation of selenium on fresh and frozen spermatozoa quality of buffalo (Bubalus bubalis) bulls. Five healthy buffalo bulls (۵ ejaculates from each bull) were used. Each ejaculate was diluted at ۳۷ ˚C with tris-based extender containing ۰ (control), ۰.۵, ۱, ۲, ۴ and ۸ μg mL-۱ sodium selenite and the sperm motility and viability were evaluated at ۰ (T۰) (immediately after dilution), ۶۰ (T۱) and ۱۲۰ (T۲) min after diluting semen. In the second step, semen samples were diluted with tris-egg yolk-glycerol extender containing the same amounts of sodium selenite, cooled to ۴ ˚C, equilibrated and semen parameters (motility, viability, membrane integrity and DNA damage) were estimated. Then, the semen was packed in ۰.۵ mL French straws and frozen in liquid nitrogen. Later, the semen was thawed and analyzed for the same parameters, as well as total antioxidant capacity. Results showed that addition of ۱ and ۲ μgmL-۱ selenium to the semen extender significantly increased the sperm motility of fresh and equilibrated semen compared to the control without affecting other parameters. However, in frozen-thawed semen, extenders containing ۱ and ۲ μg mL-۱ selenium significantly improved sperm motility, viability, membrane integrity and semen total antioxidant capacity and also resulted in lower DNA damaged sperms. In this study selenium supplementation of semen extender of ۴ and ۸ μg mL-۱ had deleterious effects on sperm parameters as early as the samples were prepared for freezing.
کلیدواژه ها:
نویسندگان
Kamran Dorostkar
Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Sayed Mortaza Alavi-Shoushtari
Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Aram Mokarizadeh
Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran