Effects of in vitro copper sulphate supplementation on the ejaculated sperm characteristics in water buffaloes (Bubalus bubalis)

This study was carried out to investigate effects of copper sulphate (CuSO۴) additive to semen extenders on sperm parameters: progressive motility, viability, membrane integrity and DNA damage, after semen dilution and cryopreservation. Semen samples of ۵ buffalo bulls of ۳-۵ years old were collected at ۵ different occasions during the autumn ۲۰۱۱. A total number of ۲۵ samples were used in each examination. Sperm progressive motility and viability were measured at ۰ (T۰), ۶۰ (T۱) and ۱۲۰ (T۲) min after diluting semen in tris-citric acid extender containing ۰ (control), ۰.۰۰۴, ۰.۰۰۸, ۰.۰۱۶, ۰.۰۳۲ and ۰.۰۶۴ mg L-۱ CuSO۴. Later, semen was diluted in a tris-citric acid-egg yolk-glycerol extender containing the same amounts of CuSO۴, cooled to ۴ ˚C and kept refrigerated for ۴ hr to equilibrate, sperm progressive motility, viability, membrane integrity and DNA damage were estimated. Then, semen was packed in ۰.۵ mL French straws and frozen in liquid nitrogen. Later, the frozen semen was thawed in ۳۷ ˚C water bath for ۳۰ sec, and the same parameters as well as total antioxidant capacity (TAC) of the frozen-thawed semen were estimated. The results showed that copper additive at the rate of ۰.۰۳۲ mg L-۱ gives a better protection of sperms through the process of dilution, equilibration and freeze-thawing than that in control and other Cu concentrations, while ۰.۰۶۴ mg L-۱ CuSO۴ had deleterious effect on the sperm.

کلیدواژه ها:

Buffalo ، copper sulphate ، Semen extender