The Effect of Interval and Continuous Training on Angiogenesis Factor in Type۲ Diabetic Wistar Rats

Objective: The equilibrium of angiogenesis stimulus agents and angiogenesis inhibitory agents is an important factor in the increase of diabetic cardiomyopathy. This research aimed to survey the result of eight weeks of high-intensity interval training (HIIT) and moderate continuous (MICT) on the myocardium angiogenesis agent and histological alterations within male diabetic rats. Materials and Methods: ۳۲ male Wistar rats did casually selected within ۴ groups: health without exercise control, diabetic without exercise (D), D + HIIT, and D+ MICT groups. Diabetes type ۲ produced with high-fat food for two weeks and an only dose of STZ. After approval of type ۲ diabetes, subjects did direct to HIIT (۹۰ -۹۵ percent of VO۲max), and MICT (۵۰ to ۶۵ percent of VO۲max) exercise program five times per week during eight weeks. Western blotting methods were utilized for the exposure of protein synthesis of Vascular Endothelial Growth Factor (VEGF), Transforming Growth Factor-beta (TGF-β۱), and Matrix Metalloproteinase-۹ (MMP۹) in the left ventricle. Besides, the base and ultimate blood glucose were estimated. Histological alterations assessed utilizing H&E and Masson’s trichrome staining. Results: The installation of diabetes develops TGF-β۱ (P-value= ۰.۰۰۱) and reduces MMP۹ (P-value= ۰.۰۰۲) and VEGF (P-value= ۰.۰۰۲). But, eight weeks of MICT enhanced MMP۹ (P-value= ۰.۰۰۲) and VEGF (P-value= ۰.۰۰۲), but the volume of TGF-β۱ (P-value= ۰.۰۰۱) diminished significantly. Furthermore, the MICT did improve better than of HIIT in improving angiogenesis agents and adjust body mass, plasma glucose in the rats with diabetes. Conclusion: Not only eight weeks of interval and continuous exercise enhanced levels of MMP۹ and VEGF, but also revealed a notable reduction in TGF-β۱. Additionally, both training diminishes body weight and blood sugar.

کلیدواژه ها:

Diabetes type ۲ ، Diabetic cardiomyopathy ، Fibrosis ، MICT training