Study of Serum Paraoxonase and High Density Lipoprotein Fractions in Diabetes

Objective: Significant alteration in lipid profile and antioxidant system occurs in response to diabetes mellitus (DM). Paraoxonase (PON) is a family of three enzymes PON۱, PON۲ and PON۳ associated with high density lipoprotein (HDL). The HDL in human plasma consists of two main sub-fractions HDL۲C and HDL۳C. We studied the HDL subclasses and HDL associated enzyme paraoxonase with respect to diabetes. Materials and Methods: The study was conducted in a tertiary care referral hospital in India. A total of ۸۰ subjects were included in the study. Lipid profile, PON۱ arylesterase (ARE), PON۱ lactonase (LACT) and HDL fractions were estimated. Regression analysis was applied. Results: PON۱ ARE, LACT and HDL fractions are found to be decreased among cases than in controls. PON۱ ARE & LACT showed negative correlation with blood glucose levels and HDL ۳C while positive correlation with HDL ۲C. Conclusion: PON۱ ARE and PON۱ LACT activities reduction are due to increased oxidative stress. PON۱ as well as HDL fraction levels are oxidative stress subjects. Among the HDL fractions, HDL۲C is the more variable fraction and reflects changes in HDL. The study suggested that the protective role of total HDL against oxidative damage and complications is mainly mediated through HDL۲C fraction.

کلیدواژه ها:

PON۱ ، Arylesterase ، Lactonase ، HDL۲ ، HDL۳