Background: Silybum marianum is considered as one of the valuable medicinal plants. This study focused on maximum recovery of total extractable compounds (TEC), anti-radical capacity (AC), and silybin content as a major phenolic compound using hydroethanolic solvent system (HSS). Methods: The HSS consisted of different ratios of ethanol (EtOH) and water (H۲O). The AC was determined using spectrophotometric analysis. High-performance liquid chromatography was used for analyzing the silybin content. The cells morphology and the mass transfer kinetics were investigated using scanning electron microscope and Peleg model respectively. Results: Based on the results, the highest TEC (۱۵۴.۶۰ ± ۲.۴ mg/g), AC (۳۹.۱۹ ± ۰.۳۰ of % DPPHsc and ۲۸.۱۵ ± ۰.۵۱ of % OHsc), and silybin (۴۴۰۰ ± ۳۳.۰۰ mg/ kg) were obtained using HSS (۸۰%v/v of EtOH). Moreover, high correlation was found between AC and silybin content. Mass transfer kinetics was successfully explained using Peleg model (R۲ = ۰.۹۹۷۰, RMSE = ۲.۲۷۴, and E = ۱.۲۲%). Further, intense cellular disruption during HSS caused accelerated release of bioactive compounds. Conclusion: The HSS composed of ۸۰% v/v of EtOH was found as an efficient system for successful recovery of bioactives which could be introduced as a natural ingredient for food and pharmaceuticals industries.

کلیدواژه ها:

Hydroethanolic solvent system ، Silybin ، Silybummarianum ، Spectrophotometric analysis ، Scanning electron microscope