Magnesium nano particles as an efficient probe followed by dispersive liquid-liquid microextraction and spectrofluorimetry for the determination of gemfibrozil

Magnesium nano particles assisted dispersive liquid-liquid microextraction (DLLME) combined with spectrofluorimetry was applied to the extraction, pre-concentration and analysis of gemfibrozil. Microextraction solvent and disperser solvent containing nano particles are directly injected into an aqueous solution containing TC. After centrifuging, phase separation is performed by sedimenting the fine droplets of the microextraction solvent on the bottom of a test tube. The settled phase is transferred into a fluorometer for the determination of gemfibrozil at excitation/emission wavelengths of 375/438 nm. Under the optimized experimental conditions, the method provides a linear dynamic range of 0.2 to 100 ng mL-1, a detection limit of 0.06 ng mL-1, and a relative standard deviation of 3.0%. The method was successfully applied to pharmaceutical formulations and human urine. The results were validated by recovery test and by comparison with other methods, and were found to be highly satisfactory

کلیدواژه ها:

Gemfibrozil ، Spectrofluorimetry ، Dispersive liquid-liquid microextraction ، Pharmaceutical ، Urine