Combination Effect of Rotator Cuff Repair with Secretome-hypoxia MSCs Ameliorates TNMD, RUNX۲, and Healing Histology Score in Rotator Cuff Tear Rats

Objectives: In order to treat a rat model of rotator cuff rupture, this work concentrated on the expression of TNMD and RUNX۲, followed by rotator cuff repair and secretome-hMSCs.Methods: A total of thirty ۱۰-weeks-old male Sprague–Dawley rats were separated into five groups randomly, RC on week ۰, lesion treated with a rotator cuff repair and saline (RC + NaCl group, n = ۶) for ۲ and ۸ weeks, and lesion treated with a rotator cuff repair and secretome-hMSCs (RC + secretome-hMSC group, n = ۶) for ۲ and ۸ weeks. The supraspinatus and infraspinatus muscle–tendon units were obtained for histological and biomechanical investigation at ۰, ۲ and ۸ weeks following injury.Results: The findings showed that, in comparison with the RC + NaCl group, secretome-hMSCs significantly improved tendon repair by upregulating TNMD and RUNX۲ expression and histology score.Conclusion: Combining Secretome-hypoxia MSCs with RC healing may help rats with rotator cuff tears. Level of evidence: IV

کلیدواژه ها:

Mesenchymal stem cells ، Rotator cuff tear ، RUNX۲ ، Secretome ، TN MD