Optimizing culture conditions and supernatant enzyme activity in a novel isolated strain of Kocuria sp.

The L-Asparaginase enzyme is an anti-cancer agent used for the treatment of certain lymphomas and leukemias, such as acute lymphoblastic leukemia (mainly in children), reticulum sarcoma, Hodgkin's disease, acute myelocytic leukemia, acute myelomonocytic leukemia, chronic lymphocytic leukemia, lymphosarcoma, and melanosarcoma. The anti-cancer properties of the enzyme are due to its ability to catalyze the hydrolysis of asparagine to aspartic acid and ammonia. The production of this enzyme depends on various factors, such as the percentage of carbon and nitrogen, the pH of the culture medium, the temperature, the fermentation time, and the amount of aeration.In this study, a bacterial strain of the genus Kocuria sp. was isolated from the soil, which could produce the asparaginase enzyme. To obtain optimal conditions for enzyme production, the bacterial culture medium was placed at different temperatures, and enzyme assays were performed at various times of growth. Additionally, to determine the optimal conditions for enzyme activity, the supernatant without bacteria was placed at different temperatures and pH, and the enzyme activity was measured using the nesslerization method. The results showed that the highest enzyme production occurred after ۴۸ hours of growth in liquid-modified Schlegel's synthetic medium at an optimum temperature of ۳۰ degrees Celsius for the growth. The optimum temperature and pH for enzyme activity of the supernatant without bacteria were ۳۰ degrees Celsius and ۸, respectively.

کلیدواژه ها:

L-asparaginase ، optimization ، leukemia ، Kocuria