توسعه ریز استخراج پخشی مایع-مایع برای تعیین اسپکتروفتومتری پنی سیلین جی در شیر و نمونه های زیستی

Background: This study developed a dispersive liquid-liquid microextraction technique followed by spectrophotometric analysis for preconcentrating/determining penicillin G benzathine and penicillin G potassium. Methods: The procedure was based on forming a color complex of penicillin G with bromothymol blue and its extraction by DLLME. The univariate method was employed for optimizing the parameters. Results: Optimized conditions for extracting benzathine and potassium penicillin G were ۱.۵ mL methanol and acetone as dispersive solvents; ۳۰۰ μL CHCl۳ and ۲۵۰ μL CCl۴ as extracting solvents; pH ۴ and ۳; ۰.۸ mL and ۰.۹ mL bromothymol blue (۱۰-۳ M), and NaCl ۰.۸ M for both analytes. The calibration curves were linear over the ranges ۵۰-۷۵۰ and ۸۰۰-۹۵۰ IU mL-۱ for analyzing benzathine, and ۸۰۰-۱۶۰۰ and ۱۷۵۰-۶۵۰۰ IU mL-۱ for potassium penicillin G. The relative standard deviations and detection limits were obtained to be ۶.۵۳% and ۰.۱۰۱ IU mL-۱ and ۴.۶۵% and ۰.۱۴۹ IU mL-۱ for determining benzathine and potassium penicillin G, respectively. Conclusion: The proposed method was employed for extracting and determining penicillin in human plasma, urine, orange juice, and milk, with acceptable recovery values of ۹۴.۳-۱۰۲%.

کلیدواژه ها:

Penicillin G benzathine ، Penicillin G potassium ، Dispersive liquid-liquid Microextraction ، Spectrophotometric determination ، Biological Samples ، پنی سیلین جی بنزاتین ، پنی سیلین جی پتاسیم ، ریز استخراج پخشی مایع-مایع ، تعیین اسپکتروفوتومتری ، نمونه های زیستی