Matrix Metalloproteases ۸ Polymorphism as Risk Factor for Rotator Cuff Tear

Objectives: Rotator Cuff Tear (RCT) is a multifactorial disease, but an important one is the increased collagen degradation that would lead to a higher chance of tear. MMP-۸ is a protein that degrades type I collagen, and it is known that MMP-۸ has a polymorphism in which a T allele in the gene promoter region increases its transcription activity. This study aims to investigate the association between MMP -۸ polymorphism g.-۷۹۹ C>T (rs۱۱۲۲۵۳۹۴) and RCT.Methods: To do that, we collected DNA samples from buccal epithelial cells of ۱۲۸ patients (separated into RCT group and control group in a proportion ۱:۱) and genotyped the DNA using PCR. The statistical analyses were done using the ARLEQUIN Version ۲.۰, and the data normality was tested with the Shapiro-Wilk test.Results: The results showed a significantly higher frequency of T/T genotype in the test group (۲۹% in the control group and ۳۹% in the test group, p=۰.۰۴۱۷), and that would represent a risk factor for increased collagen degradation.Conclusion: The MMP-۸ g.-۷۹۹ C>T (rs۱۱۲۲۵۳۹۴) SNP was associated with RCT. With the description of a new risk factor, future research can be done to analyze how to prevent RCT or develop new treatment strategies since the disease's failure index is currently high. Level of evidence: II

کلیدواژه ها:

Genetic polymorphism ، Metalloproteases ، Risk Factor ، Rotator cuff tear