Evaluation of the effect of curcumin-ciprofloxacin encapsulated chitosan nanoparticles on Pseudomonas aeruginosa

Pseudomonas aeruginosa is a gram-negative bacterium that spreads widely in the environment, usually living in soil, water, plants and humans. P. aeruginosa is an opportunistic pathogen that is one of the common causes of hospital infections. Ciprofloxacin is a broad-spectrum antibacterial drug that is used against most gram-negative bacteria. Curcumin, is a natural yellow pigment obtained from turmeric, has been identified as a potent antibacterial agent to inhibit the growth of P. aeruginosa, making it a good candidate for treating inflammatory diseases. Chitosan is a linear polysaccharide that plays a role in drug delivery. Due to biomedical properties of curcumin and chitosan, in this work chitosan nanoparticles containing curcumin and ciprofloxacin were synthesized and its antibacterial activity on clinical isolates of P. aeruginosa was investigated. P. aeruginosa strains were isolated from clinical specimens and identified by diagnostic tests including Gram staining, growth in Triple Sugar Iron agar (TSI) and cetrimide agar media, and catalase assay. The antibiotic resistance pattern was determined by the disk diffusion method and the Minimum Inhibitory Concentration (MIC) of nanoparticles and ciprofloxacin against P. aeruginosa was determined by broth microdilution assay According to the results, the bacterial strains were Gram negative, catalase positive and non-fermentative rods able to grow in cetrimide agar(۱). curcumin chitosan nano particle exhibited antibacterial activity against the selected pathogenic bacteria. Also a synergistic interaction between curcumin chitosan nanoparticles and ciprofloxacin against P. aeruginosa was observed by disk diffusion assay. The MIC of curcumin- ciprofloxacin encapsulated chitosan nanoparticles for P. aeruginosa strains ranged ۸۰-۱۶۰ µg/ml . In this study we concluded that treatment with curcumin chitosan nanoparticles and ciprofloxacin can inhibit P. aeruginosa and may be a promising treatment for P. aeruginosa infections(۲).

کلیدواژه ها:

Curcumin ، Chitosan ، Pseudomonas Aeruginosa ، Ciprofloxacin ، Nanoparticles