Performance analysis of polymerase chain reaction in relation to rapid diagnostic test (RDT) kit (SD bio line malaria ag p.f (۰۵fk۵۰)) for malaria diagnosis
سال انتشار: 1401
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 92
فایل این مقاله در 11 صفحه با فرمت PDF قابل دریافت می باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_CMBR-2-4_005
تاریخ نمایه سازی: 7 شهریور 1402
چکیده مقاله:
Malaria control and its elimination heavenly depend on successful and reliable diagnosis using recommended diagnostic techniques. These available techniques often have certain peculiarities and mode applications, thus making them have different levels of performance and accuracy. Therefore the aim of this study was to evaluate the performance of PCR in relation to Rapid Diagnostic Test Kit (SD Bio line Malaria Ag P.f (۰۵fk۵۰)) in malaria diagnosis. A total of ۲۰۰ blood samples were collected from the consented study subjects using the vein puncture technique and analysed using PCR and RDTs. Plasmodium falcifarum’s DNA was extracted using Quick-DNA™ Miniprep Plus Kit with catalog number D۴۰۶۹. ۱۸SrRNA gene of Plasmodium falciparum from chromosome ۱۳ was amplified using the two primers. For the RDTs technique, the SD Bio line Malaria Ag P.f (۰۵fk۵۰) test kit was used. Malaria prevalence of ۱۰۶(۵۳.۰%) and ۱۳۲(۶۶.۰%) were recorded using PCR and RDTs respectively. The PCR demonstrates an overall accuracy of ۰.۵۳ with sensitivity and specificity values of ۵۶.۰۶ and ۵۲.۹۴% respectively. The negative and positive predictive values were ۶۹.۸۱ and ۳۸.۳۰% respectively. PCR demonstrated a good level of performance and is therefore recommended as an effective diagnostic tool for malaria, especially in patients where the parasite density/parasitaemia level is very low.
کلیدواژه ها:
نویسندگان
Ismail Muhammad
Department of zoology, Gombe State University, Gombe, Nigeria
Pukuma Micah Sale
Zooology Department, Faculty of Science, Modibbo Adama University, Yola, Adamawa Sate, Nigeria
Tanko Mohammed
Department of Biomedical and Pharmaceutical Technology, Federal Polytechnic, Mubi, Adamawa Sate, Nigeria
Saleh Miyim
Department of General Studies, College of Nursing and Midwifery, Gombe state, Nigeria
Asiya Usman
Preliminary and general Studies Department, Federal College of Horticulture Dadin Kowa, Gombe State, Nigeria
مراجع و منابع این مقاله:
لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :