Inhibitory Effect of Supernatant and Lysate of Saccharomyces cerevisiae on Expression of exoA Gene of Pseudomonas aeruginosa

Background and Aim: Pseudomonas aeruginosa is an important ubiquitous and especially common pathogen in the hospital. Exotoxin A that encoded by exoA gene has a role in pathogenesis of this bacterium. Today, probiotics are widely used in the treatment and prevention of diseases. The present study aimed to study the Saccharomyces cerevisiae S۳ effect on the expression of exoA gene. Materials and Methods: S. cerevisiae S۳ supernatant and lysate were prepared. Subminimum inhibitory concentrations (sub-MIC) of extracts were used to P. aeruginosa PAO۱. The level of exoA expression was measured with real-time PCR method. Results: Lysate extract had a reducing effect on toxin gene expression, but unlike lysate, supernatant had an increasing effect on gene expression. Conclusion: We demonstrated that S. cerevisiae S۳ had an inhibitory effect on Exotoxin A virulence factor of P. aeruginosa. We suggest doing more experiments on the effect of S. cerevisiae on other virulence factors of P. aeruginosa and pathogens.

کلیدواژه ها:

Exotoxin A ، Probiotics ، Pseudomonas aeruginosa ، Saccharomyces cerevisiae ، اگزوتوکسین A ، پروبیوتیک ها ، سودوموناس آئروژینوزا ، ساکارومایسس سرویزیه