Effects of temperature and pH on β- gludosidases activity in Colorado potato beetle Leptinotarsa decemlineata( Col.: Chrysolellidae) midgut.

The study of insect digestive enzymes, especially in some important pests like Colorado potato beetle,L. decemlineata Say which is a serious constraint in a wide area of potato production including Iran,could be an important step in the development of a useful control strategy. Glucosidases have a main rolein the final stages of carbohydrate digestion. Digestive β glucosidases are important for the hydrolysis ofdi- and oligo-β-saccharides derived from hemicelluloses and cellulose in diet. In this research, someenzymatic properties of β-glucosidases obtained from midgut of L. decemlineata were determined .Enzyme was highly active in PH 4-6.Also, the results showed that the optimal pH and temperature for β-glucosidase activity in midgut were 5 and 50° C respectively.

کلیدواژه ها:

midgut ، Colorado potato beetle ، βglucosidase ، digestion