Orthokine therapy is an injection therapy that strengthens the body's natural defense mechanisms against inflammation to reduce pain. And also helps to improve function. Orthokine is an intra-articular Autologous Conditioned Serum (ACS or autologous conditioned serum). Orthokin’s usage may have a beneficial biological effect on arthritis pain and function. One possibility may be that therapeutic molecules help reestablish healthy joint homeostasis. ACS produces a stronger anti-inflammatory effect and modulates proinflammatory cytokines (IL-۱β and TNF-α) that are involved in cartilage destruction in OA (osteoarthritis). The main anti-inflammatory agent is called IRAP( Interleukin Receptor Antagonist Protein). This compound is obtained by taking serum from blood and incubating it. Interleukin-۱ is a powerful protein that causes inflammation. The antagonist is an agent that can prevent the effects of other substances. Therefore, IRAP is a powerful protein that prevents inflammation.The review of the articles of the last ten years provided by the sites Pubmed, ScienceDirect, Scopus, and Google scholar lets us achieve this information.Orthokine has been used as a non-aggressive treatment method to cure pain and joint injuries, and has had a positive effect in cases such as osteoarthritis (OA). By using the biological effect of ACS serum, changes in cartilage function are obvious; The pathological change of OA is the destruction of hyaline cartilage. One of the catabolic cytokines identified in osteoarthritis (OA) joints, interleukin-۱ (IL-۱) is the best-known mediator of cartilage loss. The natural inhibitor of IL-۱, IL-۱ receptor antagonist (IL-۱Ra), can potentially limit the intra-articular activities of IL-۱, as a result, the disease process is controlled. According to mentioned reviews, this treatment method can be effective in the treatment of sports injuries, joint injuries, the treatment of various types of arthritis including osteoarthritis and wound healing.