Effect of hydroalcoholic extract of trigonella foenum graecum leaves on mast cell numbers and degranulation in diabetic rat wound healing

Objective: Many studies have revealed the prominent effects of mast cells in wound healing. In the present study, we assessed the probable therapeutic features of TFG hydroalcoholic extract on wound contraction and mast cell degranulation in experimental diabetic rats.Method: Skin wounds were generated and treatments were applied with TFG hydroalcoholic extract intraperitoneally in the sham, control and experimental groups, respectively. Animals were euthanised on days ۳, ۵, ۷ and ۱۵. ۱۶,۸۰۰ microscopic images used to count the mast cells and degranulation level (Image J software).Results: Time-lapse photography revealed that the TFG significantly (p<۰.۰۵) increased the total number of mast cells in all experimental groups on days ۵ and ۷. The count of grade III (degranulated) mast cells was reduced significantly (p<۰.۰۵) on days ۵ and ۷ in experimental groups.Conclusion: The rate of wound healing was accelerated considerably following TFG hydroalcoholic extract administration.

کلیدواژه ها:

trigonella foenum graecum ، wound healing ، Diabetes