۳D bilayer skin bioconstruct for scarless healing of ۳rd degree burns in rats

A third-degree burn is one of the main clinical problems that cannot be repaired spontaneously. Herein, the acellular dermal matrix (ADM) was prepared by the decellularization of calf skin chemically and enzymatically. Copper- and zinc-doped bioactive glasses as well as electrospun nanofibers loaded with PL were synthesized and characterized in previous studies. Finally, a third-degree burn model was created in male Wistar rats and then treated with the bilayer construct. The results revealed that the ADM preserved its integrity and structure, while the residual DNA of ADM was reduced by more than ۹۵% compared to intact skin. Histological and immunohistochemistry staining of grafted bilayer construct showed increased re-epithelialization, angiogenesis, and collagen deposition compared to the control group. The prepared bilayer scaffold was able to improve the repair and regeneration of the damaged site after ۲۱ days in the rat animal model.

کلیدواژه ها:

Third degree burns ، Decellularized dermis ، Electrospun nanofibers ، Bioactive glasses