Chitosan and Nano Silver Suspension in Wound Healing of Oral Candidiasis in Piglet Infected

Introduction: Oral candidiasis (OC) is a common opportunistic fungal infection. The most common Candida species associated with oral candidiasis is Candida albicans. There are different types of clinical manifestations. The thrush (pseudomembranous candidiasis) is the most common form. Nystatin is the most widely used topical agent for the treatment of OC. Recently, studies indicated notable drug resistance which causes unsuccessful treatmentprocedures. So, new alternative replacement drugs are required to increase the effect of nystatin on resistant Candida isolates.Due to the biodegradable and nontoxic properties of chitosan particles, it may be a proper choice in clinical applications.The purpose of this study is to determine the activity of nanosilver suspension and chitosan against resistant Candida albicans in wound healing of oral candidiasis in piglet infected. Materials and Methods: The eight male pigs were infected by C. albicans and were randomly divided into four groups of two animals each. The first group included piglets whose oral wounds were infected with nystatin-resistant Candida albicans (NRCA). In the second group, piglets with infected wounds were only dressed with chitosan (NRCA/CHIT). In the third group, piglets with infected wounds were dressed in chitosan and nano silver (NRCA/CHIT/NS). In the fourth group, the wounds were created with no infection.Results: There were significant differences in comparisons of group III and other groups, particularly in terms of the presence of white spots on the tongue, throat, and other areas of the mouth. In microscopic tests with methylene blue staining, the number of Candida yeast and pseudohyphae decreased significantly (P <۰.۰۵).Conclusions: According to the importance of nystatin resistance in OC, the treatment of the disease seems to require more complicated procedures. The final results of this study indicate that final comparisons between groups of infected piglets with different dressed antifungal particles determine that using chitosan as a particle to nystatin can decrease the effects of OC more significantly. Therefore, with considerable demonstrated effects associated with nystatin as a complex clinical choice, it is suggested to modify this material as an appropriate treatment for OC.

کلیدواژه ها:

Oral candidiasis ، Chitosan ، Nanosilver ، Wound healing