Fabrication and evaluation of fibrin biological scaffolds from plasma-derived products with synthetic Teriparatide peptide in wound healing

ntroduction: This plasma product has known roles for medical applications. Teriparatide is a ۳۴ amino acid synthetic peptide approved by the Food and Drug Administration for the treatment of osteoporosis and wound healing. In the current study, we fabricated and evaluated the mechanical and biological properties of fibrin glue and reseah about woung healing. Methods: In this study, fibrinogen was extracted from FFP by using ethanol. Thrombin was precipitated by Ammonium Sulfate separation method and then the fibrin scaffold was fabricated by mixing of both fibrinogen and thrombin. Fibrinogen was characterized by FTIR and SDS-PAGE methods. In terms of morphology, (SEM) was also taken from the scaffold. Moreover, the rate of Teriparatide release within the scaffold was also measured by the Spectroscopic method. Viability was assessed by taking advantage of MTT assay in ۴ groups, including group ۱) AD-MSCs cells with fibrin scaffold, group ۲) AD-MSCs cells with fibrin scaffold containing Teriparatide peptide at the concentration of ۵۰ µg/ml, group ۳) AD-MSCs cells with fibrin scaffold containing Teriparatide peptide at the concentration of ۱۰۰ µg/ml, group ۴) AD-MSCs cells with fibrin scaffold containing Teriparatide at the concentration of ۱۵۰ µg/ml. Results: In this study, the gelation time of the fibrin scaffold was ۴±۰.۲ seconds. The highest infrared absorption for fibrinogen was at ۱۶۵۱〖cm〗^- wavenumber. For the fibrinogen sample, ۳ bands were observed in SDS-PAGE in ۴۶, ۵۲, and ۶۶ KDa. The porosity of the scaffold was ۹۱%. SEM images showed the morphology of the fibrin scaffold which was being porously networked. However, ۶۱% of Teriparatide released after ۵۴ hours. The viability of the scaffold in ۴ groups was ۹۴, ۸۶, ۷۱, and ۵۷%, respectively. Conclusion: Plasma-derived fibrin scaffold has suitable mechanical and biological characteristics for applying as a biological scaffold in wound healing .

کلیدواژه ها:

Fibrin glue ، Teriparatide ، Viability