Investigating the non-destructive effects of cold atmospheric plasma on the induced wound in healthy mice

Introduction: Non-thermal or cold atmospheric plasma (CAP) is a new therapy method that is effective in the field of medicine such as wound healing, oncology, dermatology and so forth [۱-۳]. According to the findings about the effect of CAP as a beneficial treatment method with low complications in accelerating wound healing, this study was conducted to investigate the safety and effectiveness of CAP on wounds in healthy mice (i.e., mice without disease).Materials and methods: Male, Wistar, ۸-week-old rats were divided into three groups (n=۵) and two wounds were created on the back of each mouse. The left wound of each mouse was considered as control and the right one was treated with plasma (Figure ۱ (A, B). G۱ and G۲ groups were treated with plasma for three and five minutes, respectively. While, in the G۳, the right wound was treated for three minutes and then both wounds were sutured. In this research, direct treatment with helium plasma has been performed and samplings were collected on days ۱, ۷ and ۲۱. Results: Pathology tests, proliferation index, neovascularization, re-epithelialization, N/C ratio and possible changes of cells in the direction of cell aggressiveness or malignancy after plasma therapy were examined. The mice were kept for ۳ months. No side effects such as weight loss and wound malignancy were observed. (Figure ۱ C, D).Conclusion: Malignancy and side effects were not observed in wound mice after plasma therapy and it can be considered a safe treatment.

کلیدواژه ها:

plasma ، cold atmospheric plasma ، non-thermal plasma ، plasma jet ، plasma therapy ، wound treatment ، wound healing ، wound ، mice