Introduction: The fundamental driver behind the development and investigation of treatment options is the significant proportion of people in statistical society who suffer from chronic wounds. One of the most recent techniques, gene therapy, has two components. the first is based on the permanent insertion of DNA, and the second is the temporary transformation and short-term expression of a gene product. We believe that a better understanding of how stem cells, especially MSCs and wound healing interact will aid in preventing scarring and promoting reparative healing.method: “Harmonizome"،"Gene Ontology and GO Annotations" ،"AmiGO ۲"،"panther"،"index" PubMed, and other publication resources, were used for searching the literature Result: advantages of using stem cell technology include its simplicity, minimal immunogenicity, and its eventual function in the physiology of wound healing. In order to cure wound repair, ۷۱ critical genes have been chosen. These genes are categorized into ۵ major areas based on their performance, including molecular function, biological process, cellular components, protein class, and pathways. Investigating the functions of the genes led to the formation of groups that demonstrated the significance of many of those genes' involvement in cellular processes, cellular anatomical entities, protein-containing complexes, transmembrane signal receptors, intercellular signal molecules, angiogenesis, and binding. This grouping enables us to comprehend that activating a particular gene results in a considerable rise in the product's concentration, rendering it unable to maintain and promote all stages of wound healing.Discussion: The next generation of tailored therapy for the treatment of
chronic wounds is being developed using induced pluripotent stem cell technology (iPSC). The benefit of this approach is that we can address each specific stage of wound healing by understanding how the genes work to quicken the healing process. we can manage "epigenetic differentiation" and eventually produce the targeted differentiation and diversity of epigenetics by using specific marks like DNA methylation and histone modification. In particular, if they shared an embryonic origin with the target cell type, it is the primary secret to directly changing one type of
somatic cell into another. we want to discuss the result of activating some genes in particular mature cells and
stem cells and use them in these novel approaches by identifying the most crucial genes.