Evaluation of a Wound Dressing Composed of Human Foreskin Fibroblast on a Bovine Collagen Sheet

Background: By far, a variety of biomaterials have been developed and investigated in terms of their suitability for the full-thickness skin defects. Collagen as a key component of extracellular matrix with attributes such as biocompatibility, biodegradability, and various functional moieties for chemical modification have attracted much attention as wound-dressing. Collagen has a very poor immune response and doesn't produce unwanted side effects. Collagen dressing can decrease the wound liquid's pH, reducing the risk of secondary bacterial colonization. One the other hand, the structure of the collagen scaffold provided a suitable platform for the transferring of fibroblast cells and facilitates their migration into the injury zone and speeds up angiogenesis in the wound area. The bovine collagen sheet )Suprasorb® C) is a protease-modulating dressing designed not only to promote a moist wound environment but also to support growth factors and inactivate proteases. This structure also improved the microcirculation and promoted angiogenesis and finally promote homeostasis in wound area. Fibroblasts have critical roles in supporting normal wound healing, involved in creating new extra cellular matrix and secreting various growth factors and cytokines that have a direct effect on epidermal proliferation and differentiation. With this background, the present study was designed to investigate the ability of Suprasorb C collagen sheet to develop a substrate for seeding, maintain and infiltration of human foreskin fibroblast (hFF) and its healing potential for full thickness skin wounds in wistar rats. Materials and Method: In this study, hFF cells were supplied by the Royan Stem Cell Bank and cultured in appropriate environment. The collagen sheet (Suprasorb® C) was cut out in dimensions of ۰.۲ × ۰.۲cm۲ and inserted into the wells of a ۲۴-well plate. The cells were loaded at the density of ۱۰۵ cells/well on the collagen sheet. The MTS assay was performed to analyze the viability and proliferation of the cells on the collagen sheet after one, two, and three days of incubation. For in vivo assessment, ten weeks wistar rats (۲۵۰-۳۰۰ gr) were used according to the approved animal protocols. Before the surgery, the rats were anesthetized by Isoflurane ventilator. After shaving the dorsal hair and disinfecting the skin, full- thickness skin with a diameter of ۲ cm were created on the side of rats and fixed with a silicone ring to impede wound contraction

کلیدواژه ها:

Human foreskin fibroblast ، Collagen sheet ، Full-thickness wound ، Wound dressing