فلور قارچی خوراک دام و آلودگی آفلاتوکسین خوراک و شیر در گاوداری های شیری شهرستان سبزوار

Afalatoxins are among the most poisoning secondary metabolites produced by different fungi species such as Aspergillus flavus and Aspergillus paraziticus. These fungi contaminate different animal feed materials including oil seeds, oil seed meals and forages.  Elimination and reduction of aflatoxines in feed using biological, chemical and physical methods of aflatroxin detoxification in large scales are not only non practical and cost efficient, but also are dangerous for animal health. Therefore, it seems continuously monitoring of animal feed and products is the best method for avoiding of human food chain contamination with these toxins. This research was conducted to investigate the feed and milk aflatoxin contamination and their relationship with feed fungi flora in some dairy cow farms. Concentrate and corn silage were sampled from ۵ traditional and ۵ industrial dairy farm among dairy farms located around the Sabzevar county, cultivation and identification of feed fungi were done. Milk samples were taken from cooling tank of each farm and transferred to lab in an ice containing flask and were centrifuged to remove fat and then stored in -۲۲°C until analysis. Milk and feed aflatoxin were measured using HPLC method. The most comon feed pollutants were Aspergilus, Penicillium, Trichoderma, Alternaria, Cladosporium and Rhizopus species. The results of HPLC and fungal culture showed a low level of aflatoxin contamination. The results of this research showed a significant negative correlation between Aspergilus contamination and yeast population in silage samples. Aflatoxin M۱ contamination was observed in ۶۰ percent of milk samples, however aflatoxin amount was lower than permissive levels in all cases. Mean Aspergilus population in concentrate and aflatoxin concentration of milk Comparison among two farm systems, showed that relative contamination in industrial farms was significantly higher than traditional farms. Results of this research showed that aflatoxin contamination of feed and milk was lower than permissive levels. Based on fungi culture results, it seems that concentrate is more susceptible to fungi and aflatoxin contamination than silage.

کلیدواژه ها:

خوراک دام ، آسپرژیلوس ، آفلاتوکسین B۱ ، شیر ، آفلاتوکسین M۱