The inhibitory effect of Iron oxide nanoparticles on biofilm formation Pseudomonas Aeruginosa clinical isolates

Biofilms are one of the mechanisms which cause antibiotic resistance in Pseudomonas Aeruginosa. Therefore, it plays a significant role in nosocomial infections. Biofilm formation allows this bacterium to adhere to the respiratory mucosal membranes and prevent antibody attachment to the bacterium. Additionally, this bacterium can grow in simple aqueous solutions, leading to the contamination of anesthesia equipment, respiratory therapy, intravenous fluids, and even distilled water in hospitals. It causes infections in hospitalized patients with extensive burns or compromised skin integrity. In recent years, researchers have been exploring new ways to inhibit bacterial biofilms to reduce the risk of infections.One promising approach is the use of nanoparticles. Nanoparticles have advantages over traditional antibiotics because they are less likely to cause antibiotic resistance. Most nanoparticles work by preventing biofilm formation, which allows the immune system to more effectively combat the bacteria. Unlike antibiotics, which only eliminate surface bacteria of the biofilm, super magnetic nanoparticles affect biofilm synthesis at different stages. This means that they can penetrate deeper into the biofilm and target the bacteria that are most resistant to antibiotics. For this reason's aim of present study was to investigate the inhibitory effect of super magnetic Iron oxide nanoparticles on P. Aeruginosa clinical isolates and effect on inhibition formation bacterial biofilm.In the first step, super magnetic Fe۳O۴ nanoparticle powder synthesized by Co-precipitation method and culture was prepared with bacteria suspension (P. aeruginosa, ۱۰۶ CFU/mL in Luria-Bertani Broth solution) and various concentrations of Fe۳O۴ to ۹۶-well flat-bottom tissue culture plates (TCP) incubation at ۳۷ ºC for ۴۸ hours. Crystal violet (۰.۱%, w/v) and ethanol (۹۵%) were used to fix and color bacterial biofilms, then the optical densities (OD) were assessed at ۵۹۰ nm using a microplate reader (model CS, Biotec).The result has shown that Fe۳O۴ nanoparticles are effective in inhibiting biofilm formation of P. aeruginosa. This is significant because P. aeruginosa is one of the most important bacteria that forms biofilms and is a common cause of nosocomial infections.Additionally, they can be targeted to specific areas of the body where biofilms are present. This makes them a promising option for preventing and treating infections caused by biofilm-forming bacteria.In conclusion, the use of Fe۳O۴ nanoparticles to inhibit biofilm synthesis is a promising approach for preventing and treating infections caused by biofilm-forming bacteria such as P. aeruginosa. Further research is needed to explore the potential of these nanoparticles in clinical settings.

کلیدواژه ها:

Biofilm ، Pseudomonas aeruginosa ، super magnetic ، nano particle ، Fe۳O۴