The evaluation of connection between integrons class I and II and ESBL-producing and Non-ESBL klebsiella pneumoniae isolated from clinical samples, Kerman

Background and Aim: Genes encoding ESBL are usually located on conjugative plasmids, although ESBL genes are found within integron-like structures. The main purpose of current study was to detect a relationship between integrons class I and II and ESBL-producing, Non-ESBL Klebsiella pneumoniae.. Materials and Methods: In the current study, ۵۸ samples of K. pneumoniae were collected from urine, blood, and wound in Kerman hospitals. Then antibiotic susceptibility test was carried out by using disc diffusion method. ESBLs producing K. pneumoniae determined using phenotypic confirmatory test. Detection of bla-TEM, bla-CTXM, bla-SHV, intI, and intII genes was done by PCR. Eventually, data analysis were performed using SPSS-۲۲. Results: Antibiotic susceptibility test was showed that antibiotic resistance patterns are different between ESBL-producing and Non-ESBL K. pneumoniae. Effective antibiotic against K. pneumoniae isolates was imipenem (۵۷.۸%) and ۵۵ isolates (۹۴.۸%) were resistance to ampicillin which more than others antibiotics. Also, ۳۵ (۶۰.۳%) isolates were produced ESBL-enzymes. ۳۷ (۶۳.۷%) isolates included intI while just ۳ (۵.۱%) isolates had intII genes. In this study, we observed a significant association between ESBL and integron class I. Conclusions: High prevalence of integron and ESBLs in K. pneumoniae demonstrated that following health recommendations in hospitals and prescribing correct antibiotics can prevent the antibiotic resistance in the future.

کلیدواژه ها:

Klebsiella pneumoniae ، Disc diffusion ، Imipenem ، ESBL ، کلبسیلا پنومونیه ، دیسک دیفیوژن ، ایمی پنم ، ESBL