Association of Arachidonic Acid, Eicosapentaenoic Acid and Serum Amyloid A Levels in Women with PCOS

Background & Objective: PCOS is a multifactorial disorder characterized by inflammation caused by unknown reasons. This study aimed to investigate the Arachidonic acid (AA), eicosapentaenoic acid (EPA) levels and Serum amyloid A (SAA) protein and gene expression of follicular fluid in women with PCOS. Materials & Methods: The study included ۳۰ PCOS and ۳۰ healthy women. The individuals were selected after clinical examination and the inclusion criteria. During the puncture and after removing oocytes, follicular fluids were collected from individuals. The SAA protein concentration was determined by the ELISA method. Arachidonic acid, eicosapentaenoic acid levels were determined using GC method and the statistical software SPSS ۱۶, and the p value less than ۰.۰۵ was considered as significant. Results: There was a significant difference in metaphase II oocytes (M۲) (p =۰.۰۰۱), number of Embryo (p =۰.۰۱۴), Estradiol (p =۰.۰۲۵), FSH (۰.۰۴۰), LH (p =۰.۰۳۵) and Prolactin (p =۰.۰۲۳) levels between two groups. The concentration of SAA protein level and gene expression shows a significant increase in the patient group compared to the control group (p <۰.۰۵). The results showed that AA level was significantly increased in the patient group (۲۸۴.۱۲ ng/mL) compared to the control group (۱۷۶.۲۳ ng/mL) (p =۰.۰۰۱) while, there was a significant decrease (p =۰.۰۰۱) in EPA level in the patient group (۲۴.۵۶ ng/mL) compared to the control group (۳۳.۴۸ ng/mL). Conclusion: It appears that higher EPA levels and lower follicular fluid AA concentrations reduce SAA levels and thus may increase follicular maturation in women with PCOS.

کلیدواژه ها:

PCOS ، Follicular maturation ، Serum amyloid A ، Fatty Acids