Expression Profiles of P۵۳, Caspase-۳ And Bcl-۲ in Patients Undergoing Congenital Heart Corrective surgery: Combined Effects Of Anesthesia And Surgery

Background: Apoptosis is a physiological programmed cell death necessary for the development and cellular homeostasis. Dysregulation of apoptosis pathways leads to several diseases such as cancer, autoimmune and immunodeficiency diseases, and neurodegenerative disorders. Materials and Methods: The current study included ۵۶ patients (۲۹ males and ۲۷ females), undergoing corrective heart surgery operations, categorized into ۳ groups: group A included ۲۳ patients with atrial septal defect (ASD); group B included ۱۵ patients with ventricular septal defect (VSD), and group C included ۱۵ patients with Fallot tetralogy. Biochemical assays of apoptotic (P۵۳ and caspase ۳) and antiapoptotic markers (Bcl-۲) using colorimetric and ELISA assay kits were performed on all included patients twice, preoperative and ۲۴ hours postoperative. Results: There was no statistically significant difference in the preoperative levels of Bcl-۲, Caspase-۳, and P۵۳ between the three groups. While the significant difference was found when comparing the preoperative and postoperative levels of the previous markers in the same group and between groups (p˂۰.۰۵ for all). Conclusion: Both apoptotic and antiapoptotic pathways are activated during congenital heart corrective surgeries, increased markers directly related to the duration of anesthesia exposure.

کلیدواژه ها:

P۵۳ ، Caspase-۳ ، Bcl-۲ ، Congenital Heart Surgery ، Apoptosis