Fabrication and Evaluation of Spironolactone‑Loaded Nanostructured Lipid Carries for Cardiac Tissue Regeneration

سال انتشار: 1399
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 122

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شناسه ملی سند علمی:

JR_JMSI-10-4_005

تاریخ نمایه سازی: 28 تیر 1402

چکیده مقاله:

Background: Spironolactone (SP) is a lipophilic aldosterone receptor antagonist that few studies have reported its effect on cardiac remodeling. In addition, fewer researches have considered its influence on cardiomyocyte viability and potential benefits for myocardial tissue remodeling. Method: In this study, stearic acid (SA) (solid lipid) and oleic acid (OA) (liquid lipid) were utilized to produce nanostructured lipid carries (NLCs) (various ratios of SA to OA and water amount, F۱: ۸۰:۲۰ [۳۰ ml water], F۲: ۸۰:۲۰ [۶۰ ml water], F۳: ۷۰:۳۰ [۳۰ ml water], and F۴: ۷۰:۳۰ [۶۰ ml water]) containing SP and their particle size, polydispersity index, zeta potential, entrapment efficiency, and release profile were measured. The purpose of encapsulating SP in NLCs was to provide a sustain release system. Meanwhile, ۳‑(۴,۵‑dimethylthiazol‑۲‑yl)‑۲,۵‑diphenyltetrazolium bromide assay with different concentrations of SP‑loaded NLCs (SP‑NLCs) was conducted to evaluate the cytotoxicity of the NLCs on rat myocardium cells (H۹C۲). Results: Increase of oil content to ۱۰ wt% reduced the particle size from ۴۸۶ nm (F۱) to ۲۰۵ nm (F۲). Zeta potential of the samples at around −۱۰ mV indicated their agglomeration tendency. After ۴۸ h, SP‑NLCs with the concentrations of ۵ and ۲۵ µM showed significant improvement in cell viability while the same amount of free SP‑induced cytotoxic effect on the cells. SP‑NLCs with higher concentration (۵۰ µM) depicted cytotoxic effect on H۹C۲ cells. Conclusion: It can be concluded that ۲۵ µM SP‑NLCs with sustain release profile had a beneficial effect on cardiomyocytes and can be used as a mean to improve cardiac tissue regeneration.

نویسندگان

Mehrzad Falak

Department of Biomedical Engineering, Faculty of Engineering, University of Isfahan

Mehdi Mehdikhani

Department of Biomedical Engineering, Faculty of Engineering, University of Isfahan

Jaleh Varshosaz

Department of Pharmaceutics, Novel Drug Delivery Systems Research Centre, School of Pharmacy, Isfahan University of Medical Sciences

Batool Hashemibeni

Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran