Effect of glycyrrhizin and its derivatives on integrity of human red blood cells
سال انتشار: 1400
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 111
فایل این مقاله در 8 صفحه با فرمت PDF قابل دریافت می باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_HERM-11-4_012
تاریخ نمایه سازی: 28 تیر 1402
چکیده مقاله:
Introduction: The first and most prevailing cells that glycyrrhizin (GL) and glycyrrhetinic acid (GA) encounter are red blood cells (RBCs). However, what follows this event is poorly understood. This study aims to evaluate the effect of GL and its derivatives on the integrity of human RBCs.
Methods: The integrity of human RBC was assessed under normal isotonic conditions and following osmotic and nystatin-induced colloid-osmotic stress by measuring the amount of hemoglobin released. The pore size was determined by the osmotic protection method.
Results: GL was found to be virtually non-hemolytic. However, removal of the carbohydrate moiety of GL imparted significant RBC lytic activity to the cis-(beta-) but not to the trans-(alpha-) isoform of GA. The hemisuccinate radical at position C۳ (carbenoxolone) greatly diminished the hemolytic property of GA. The RBC lysis occurred by colloid-osmotic mechanism due to the formation of hydrophilic pores with the radius of ~۲.۳ nm. At the sublytic doses, the two stereo-isoforms displayed opposite effects on the osmo-resistivity of human RBC: osmoprotection for alpha-GA and osmotic sensibilization for beta-GA. Similar osmotic sensibilization was also observed for GL and carbenoxolone. The two stereo-isoforms exhibited different but not opposite weakening effects on the resistivity of the RBC to the colloid-osmotic stress induced by nystatin, a pore-former. The weakening effect was found intermediate for GL and absent for carbenoxolone.
Conclusion: Upon intestinal digestion and absorption, depending on the structure and dosage, the GL hydrolysis products interact with RBC with both beneficial and detrimental consequences.
کلیدواژه ها:
Glycyrrhizin ، Glycyrrhetinic acid ، Carbenoxolone ، Erythrocytes ، Red blood cell lysis ، Pore size
نویسندگان
Diyor Fayziev
Institute of Biophysics and Biochemistry, National University of Uzbekistan, Tashkent, Uzbekistan
Peter Merzlyak
Institute of Biophysics and Biochemistry, National University of Uzbekistan, Tashkent, Uzbekistan
Sarvinoz Rustamova
Institute of Biophysics and Biochemistry, National University of Uzbekistan, Tashkent, Uzbekistan
Ozoda Khamidowa
Institute of Biophysics and Biochemistry, National University of Uzbekistan, Tashkent, Uzbekistan- Department of Biophysics, National University of Uzbekistan, Tashkent, Uzbekistan