The effect of the healing rate of Polyvinyl Alcohol Nano-Fibers Containing Pistachio Hull Extract on healing of bone defect induced in the rat calvarium

Fracture healing is important in medicine; thus seeking new techniques with fewer side effects to improve the speed of the healing is prudent. Pistachio hull extract has strong antioxidant properties that can be used as scaffolds in nano dimensions. This study aimed to evaluate the effect of nanofibers containing pistachio hull extract on bone defect healing in rat calvaria. After preparing the pistachio hull extract (PHE), electrospun PVA/PHE nanofibrous mats were fabricated using the electrospinning method. The morphological and structural properties of the fabricated mats were characterized by scanning electron microscopy, Fourier-transform infrared spectroscopy, and X-ray diffraction. Histopathological assessments were performed on ۴۵ male Wistar rats on days ۱۴, ۲۸ and ۴۲. A ۷-mm bone defect was created in the calvaria of all rats. The animals were divided into three groups, and the defect in each group were covered with PVA/PHE, normal saline, and PVA wound dressings. Statistical analysis was performed by the Kruskal-Wallis using the SPSS software. Based on the results, no significant difference was observed between the studied groups on day ۱۴. On days ۲۸ and ۴۲, there was a significant difference between the PVA/PHE and the other two groups, while no significant difference was observed between the PVA nanofiber group and the control group. The results revealed that PVA/PHE can increase the amount of bone formation and improve the healing process in the defect created in the rat calvarium bone. Therefore, it may be considered a promising alternative for clinical administration.

کلیدواژه ها:

Calvarium ، Nanofiber ، Pistachio hull ، Rat.