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Cytotoxicity of curcumin against CD44± prostate cancer cells: Roles of miR-383 and miR-708

سال انتشار: 1402
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 237

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شناسه ملی سند علمی:

JR_AJP-13-4_008

تاریخ نمایه سازی: 14 تیر 1402

چکیده مقاله Cytotoxicity of curcumin against CD44± prostate cancer cells: Roles of miR-383 and miR-708

Objective: Cancer stem cells (CSCs) remaining in the tumor tissues after applying treatments may cause recurrence or metastasis of prostate cancer (PC). Curcumin has the promising potential to target CSCs. Here, we aim to evaluate the cytotoxic effects of curcumin on the expression of miR-383-5p and miR-708-5p and their target genes in CD44+ CSCs and CD44- non-CSCs isolated from the PC3 prostate cancer cell line.Materials and Methods: We used MTT assay to determine the optimal cytotoxic dose of curcumin on CD44± PC cells. Then, we assessed nuclear morphological changes using DAPi staining. We used Annexin V-FITC/PI to quantify apoptotic cell death. qRT-PCR was also used to detect miRNA and gene expression levels after curcumin treatment.Results: Curcumin significantly enhanced the apoptosis in both CD44- and CD44+ PC cells in a dose-dependent manner (p < 0.05). The cytotoxicity of curcumin against CD44- cells (IC50  40.30±2.32 μM) was found to be greater than that against CD44+ cells (IC50  83.31±2.91 μM). Also, curcumin promoted miR-383-5p and miR-708-5p overexpression while downregulating their target genes LDHA, PRDX3, and RAP1B, LSD1, respectively.Conclusion: Our findings indicate that curcumin, by promoting the expression of tumor suppressors, miR-383-5p and miR-708-5p, and inhibiting their target genes, induced its cytotoxicity against CD44± PC cells. We trust that curcumin could be established as a promising adjuvant therapy to current PC treatment options following more research in clinical settings.

کلیدواژه های Cytotoxicity of curcumin against CD44± prostate cancer cells: Roles of miR-383 and miR-708:

نویسندگان مقاله Cytotoxicity of curcumin against CD44± prostate cancer cells: Roles of miR-383 and miR-708

Reza Panahizadeh

Student Research Committee, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran

Mohammad Amin Vatankhah

Students Research Committee, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran

Farhad Jeddi

Department of Medical Genetics and Pathology, Faculty of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran

AmirAhmad Arabzadeh

Department of Surgery, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran

Kazem Nejati-Koshki

Pharmaceutical Sciences Research Center, Ardabil University of Medical Science, Ardabil, Iran

Ramin Salimnejad

Research laboratory for Embryology and Stem Cells, Department of Anatomical Sciences, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran

Nowruz Najafzadeh

Research Laboratory for Embryology and Stem Cells, Department of Anatomical Sciences, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran